We narrowed to 23,617 results for: CHI

1. pET-mKelly2

   Plasmid

   #119783

   Purpose

   Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly2
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-ldhGFP

   Plasmid

   #27170

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCTCON2-Aga1p

   Plasmid

   #158141

   Purpose

   Expresses Aga1p in yeast

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rab9

   Plasmid

   #31736

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab9 (Rab9 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Actin

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEZYmyc-His

   Plasmid

   #18701

   Purpose

   pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTKLP-tetA

   Plasmid

   #71325

   Purpose

   tetA landing pad template plasmid

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-EGFP-BarcodeV5

   Plasmid

   #78629

   Purpose

   GESTALT V5 integrating barcode with Puro and EGFP

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   GESTALT lineage tracing target V5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GESTALT_pX330-v1

   Plasmid

   #103061

   Purpose

   plasmid px330 with guide targeting GESTALT v1 through V5 constructs

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

    Plasmid

    #187100

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-SF-iGluSnFR
    

    Use

    Tags

    mCherry-SL2

    Expression

    Worm

    Mutation

    Promoter

    Ttx-3

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-LacI

    Plasmid

    #46394

    Purpose

    Overproduction of LacI

    Depositor

    Florian Hollfelder

    Article

    Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

    Insert

    LacI
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    silent T to C mutation at position 19

    Promoter

    arabinose promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNR220

    Plasmid

    #79541

    Purpose

    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    A118
    

    Use

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-6xHis

    Plasmid

    #87343

    Purpose

    To express EGFP-6xHis in mammalian cell

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    6xHis
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-periHisMBP

    Plasmid

    #11086

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and MBP signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Use

    Tags

    RFP

    Expression

    Plant

    Mutation

    Promoter

    AtOLE1

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p104_gRNA_Sp_CD3e

    Plasmid

    #213780

    Purpose

    Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV PRDM16

    Plasmid

    #15504

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (isoform 4) (Prdm16 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmNeonGreenHO-3xFLAG-G

    Plasmid

    #127914

    Purpose

    a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

    Depositor

    Isei Tanida

    Article

    Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

    Insert

    humanized mNeonGreen
    

    Use

    Tags

    mNeonGreen-3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL0_3 [RSF1010 ORI]

    Plasmid

    #198920

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Charlie Gilbert, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RSF1010 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-mKelly1

    Plasmid

    #119782

    Purpose

    Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p105_gRNA_Sa_CD40

    Plasmid

    #213781

    Purpose

    Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKSI-1

    Plasmid

    #51725

    Purpose

    cloning backbone

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs-DsRed Express

    Plasmid

    #22724

    Depositor

    Shinya Yamanaka

    Article

    Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

    Insert

    a variant of Discosoma sp. red fluorescent protein
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEM705 Gsq

    Plasmid

    #109356

    Purpose

    GsX chimera with Gαq C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsq chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMO9075

    Plasmid

    #117481

    Purpose

    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

    Depositor

    Judy Wall

    Article

    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

    Insert

    Aph(3')-IIa promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aph(3')-IIa promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLew100::NLS-ISceI-HA

    Plasmid

    #21299

    Depositor

    Nina Papavasiliou

    Article

    Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

    Insert

    I-SceI (SCEI )
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-myrGFP

    Plasmid

    #115502

    Purpose

    expression of myristoylated, plasma membrane targeted GFP

    Depositor

    Thomas Biederer

    Article

    Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

    Insert

    myristoylated GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only