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pSN35

Plasmid

#100120

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSN31

Plasmid

#100116

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSN32

Plasmid

#100117

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del3

Plasmid

#96913

Purpose

Murine Atoh1 with amino acids 214-305 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 214-305

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del4

Plasmid

#96914

Purpose

Murine Atoh1 with amino acids 306-347 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 306-347

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-chCAR

Plasmid

#87231

Purpose

Mammalian expression of chicken CAR

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mc2r-mod

Plasmid

#48173

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Mutation

A/G MUTATION IN ATG OF UORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Kdr-mod

Plasmid

#48177

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Mutation

A/G MUTATION IN ATG OF UORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
5mARF17 pBluescript

Plasmid

#12069

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Expression

Bacterial

Mutation

miR160-resistant version of ARF17 has five silent…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRN120

Plasmid

#160696

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

CmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S

Use

Luciferase; Gemini viral replicon, plant developm…

Promoter

AtUbi10, CmYLCV, and Nos

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-1304)

Plasmid

#74244

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-1304

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-CRM1

Plasmid

#237646

Purpose

For overexpression of mCherry-CRM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CRM1 (XPO1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1

Plasmid

#237661

Purpose

For integration of EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Synthetic, Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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