We narrowed to 5,906 results for: pcDNA 3.1

1. mVenus-hCOL1A1

   Plasmid

   #140111

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-MRGBP

   Plasmid

   #15325

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

   Insert

   MRG-binding protein (MRGBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

   Plasmid

   #163844

   Purpose

   Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mito-mGarnet2

   Plasmid

   #104310

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   mito-mGarnet2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNIR-FbGFPY58/C-PKI

   Plasmid

   #184685

   Purpose

   NIR-Fb-based PKA inhibitor

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFPY58/C-PKI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRPML1-HA

   Plasmid

   #18825

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML1 (MCOLN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FL-mCherry

   Plasmid

   #78862

   Purpose

   DULIP negative control (prey) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-MCPIP1

   Plasmid

   #127459

   Purpose

   Expressing human MCPIP1 in mammalian cells

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 4xHRE_YB TATA-sfGFP-CMV_dsRed

   Plasmid

   #117399

   Purpose

   sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRPML1-CFP

    Plasmid

    #18827

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. hTPST1

    Plasmid

    #11252

    Depositor

    Hyeryun Choe

    Article

    Choe et al Cell. 2003 Jul 25. 114(2):161-70.

    Insert

    TPST1 (TPST1 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

12. mito-mGarnet

    Plasmid

    #104309

    Purpose

    codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

    Depositor

    Ulrich Nienhaus

    Article

    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

    Insert

    mito-mGarnet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK

    Plasmid

    #62670

    Purpose

    Vector for PhiC31o mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SP-GFP-CD274-UTR

    Plasmid

    #121142

    Purpose

    Mammalian expression of GFP tagged CD274 with its own 3'UTR

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    CD274 with its own 3'UTR (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FlagMBNL3

    Plasmid

    #96901

    Purpose

    expression of Flag-tagged human MBNL3

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human MBNL3 cDNA (MBNL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Mammalian_SpCas9_Target-AID2S

    Plasmid

    #188648

    Purpose

    Expresses SpCas9_Target-AID2S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

    Plasmid

    #117401

    Purpose

    sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOVC1_TtCarH-CBD_X

    Plasmid

    #131179

    Purpose

    Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    TtCarH-CBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge