We narrowed to 18,867 results for: IRE

1. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

   Plasmid

   #183411

   Purpose

   piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   Tet-on 3G
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

   Plasmid

   #141372

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

   Plasmid

   #85146

   Purpose

   lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mRuby3-P2A-mTurquoise-MLC
   

   Use

   Lentiviral

   Tags

   mRuby3, mTurquoise

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

   Plasmid

   #141378

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

   Plasmid

   #141373

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

   Plasmid

   #235087

   Purpose

   GFP splicing reporter with FRG1 intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   custom bidirectional

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

   Plasmid

   #62458

   Purpose

   expresses his-tagged C151S human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteine 151 of Keap1 to serine

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

   Plasmid

   #141374

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

    Plasmid

    #231007

    Purpose

    Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    E4F1 aa220-242 (E4F1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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