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pBR322-MCK1.35lux

Plasmid

#53368

Purpose

expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

Depositor

Josephine Nalbantoglu

Article

Larochelle et al Gene Ther. 1997 May;4(5):465-72.

Insert

muscle creatine kinase promoter/enhancer 1.35kb

Expression

Mammalian

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-Ypet-PBD

Plasmid

#24533

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Use

Retroviral

Tags

Ypet

Expression

Mammalian

Available Since

Aug. 19, 2010

Availability

Academic Institutions and Nonprofits only
pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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KK601: pMVP (L3-L2) ecDHFR DD + polyA

Plasmid

#121790

Purpose

pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

ecDHFR Degron Domain + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_alphaTubulin_C1

Plasmid

#85046

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNAZeo(-)HSC73

Plasmid

#86030

Purpose

Expression of Heat shock chaperone 70 in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Heat-shock cognate protein 73 (HSPA8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgGFP2, sgGFP3

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_mNeonGreen_FLAG_C

Plasmid

#218073

Purpose

RESOLUTE cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pLux02

Plasmid

#78282

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_H2A_C1

Plasmid

#85052

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMCS.71/2272

Plasmid

#22732

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-MCS-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE containi…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
p406 – TDH3-Elo3-GFP11-Ura

Plasmid

#177734

Purpose

ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

Elo3 (ELO3 Budding Yeast)

Tags

GFP11 and HDEL

Expression

Bacterial and Yeast

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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