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pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

Plasmid

#129539

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-SwiChR-eYFP

Plasmid

#166604

Purpose

Encodes Cre-dependent SwiChR-eYFP under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

SwiChR-eYFP

Use

AAV

Promoter

tetracycline response element

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-27b

Plasmid

#112539

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-27b (MIR27B Human)

Use

Adenoviral

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-Y16C

Plasmid

#30372

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y16C (PTEN Human)

Expression

Mammalian

Mutation

Y16C

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
p3E-SV40-puromycin

Plasmid

#138358

Purpose

Gateway compatible 3' entry clone contains puromycin driven by simian virus 40 (SV40)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

SV40-Puromycin

Expression

Mammalian

Promoter

The simian virus 40 (SV40) promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF4b

Plasmid

#67498

Purpose

Expression of shRNA targeting human ARF4b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5a

Plasmid

#67499

Purpose

Expression of shRNA targeting human ARF5a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5b

Plasmid

#67500

Purpose

Expression of shRNA targeting human ARF5b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2 xIGF2

Plasmid

#24689

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin-like growth factor 2 (igf2-b Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFS331-USP44-catDead-HIS

Plasmid

#43903

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS

Expression

Mammalian

Mutation

C282A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Tags

GFP

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pRmHa3-dKDM4A-HA2FL2

Plasmid

#20232

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-D24Y

Plasmid

#30374

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-D24Y (PTEN Human)

Expression

Mammalian

Mutation

D24Y

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHJ42

Plasmid

#48359

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBacPAK-HFT-dKDM4A

Plasmid

#20233

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4B

Plasmid

#20234

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4B (Kdm4B Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only