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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBA2257

Plasmid

#38151

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

MBP-proMeCPA fusion protein

Tags

maltose binding protein

Expression

Bacterial

Promoter

tac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-sAMO

Plasmid

#20111

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and codon optimized amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
HP17deltaP8

Plasmid

#235447

Purpose

Helper plasmid for regulated phage transfer

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hcMYC

Plasmid

#206387

Purpose

Expresses human c-MYC in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

c-MYC (MYC Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CNOT3

Plasmid

#155467

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CNOT3 (CNOT3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(D323A/D325A)

Plasmid

#86630

Purpose

Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1(D323A/D325A) (AKT1 Human)

Expression

Mammalian

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Synthetic, Human)

Use

Gateway Cloning

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1_EHD1_438-534

Plasmid

#36459

Depositor

Yvonne Groemping

Insert

EHD1 EH domain (EHD1 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-437 from full-length constr…

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pRS513

Plasmid

#17283

Depositor

Nancy Craig

Article

Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

Insert

TnsB (tnsB E. coli transposon Tn7)

Tags

Chitin-binding domain-intein

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
979 pSG5L HA PTEN 1-393

Plasmid

#10767

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-393.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD24-T2A-GFP

Plasmid

#205456

Purpose

lentiviral plasmid for expression of human CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD24 (CD24 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.30_Gag-MCP-dpol-T2A-eGFP

Plasmid

#205538

Purpose

Express HIV Gag-MCP-dpol-T2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

HIV Gag-MCP-dpol

Expression

Mammalian

Mutation

pol frameshift sequence recoded

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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