We narrowed to 16,541 results for: puromycin

1. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

   Plasmid

   #196192

   Purpose

   human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
   

   Use

   Znf donor

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPM-D4 miR30-TRIM28 ts3

   Plasmid

   #115864

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK-PUM1-rABE-Flag

   Plasmid

   #191385

   Purpose

   Doxycycline inducible expression of PUM1-rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   PUM1-rABE (PUM1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   tet-inducible promoter

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_CMV:Flag-GeNL_SV40:PuroR

   Plasmid

   #183040

   Purpose

   Expresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   GeNL Nanolantern
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXL004-ishRNA-scramble

   Plasmid

   #36311

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of scramble
   

   Use

   Lentiviral and RNAi

   Tags

   EF1a-TetR-IRES-RFP-Puro

   Expression

   Mammalian

   Promoter

   TetO-H1

   Available Since

   July 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-FLAG-KD LKB1

   Plasmid

   #8593

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMK074: CMV QuasAr2-TS-GCaMP6f

   Plasmid

   #72303

   Purpose

   Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

   Depositor

   Adam Cohen

   Article

   Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

   Insert

   CaViar
   

   Use

   Lentiviral

   Tags

   TS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Ki67-mCherry

   Plasmid

   #183748

   Purpose

   Expresses EGFP-Ki67-mCherry

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC2-MTHFD1-3xFLAG

    Plasmid

    #217429

    Purpose

    Lentiviral overexpression of human MTHFD1

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti TurboRFP BlastR

    Plasmid

    #102343

    Purpose

    Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Tags

    N/A

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CMV:Flag-CeNL_SV40:PuroR

    Plasmid

    #183039

    Purpose

    Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_CMV:Flag-YeNL_SV40:PuroR

    Plasmid

    #183041

    Purpose

    Expresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    YeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CMV:Flag-OeNL_SV40:PuroR

    Plasmid

    #183042

    Purpose

    Expresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    OeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-TfR-mApple

    Plasmid

    #179384

    Purpose

    Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    Transferrin receptor 1 - mApple (TFRC Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_CMV:Flag-NLuc_SV40:PuroR

    Plasmid

    #183045

    Purpose

    Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    NanoLuciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4_10xUAS-MLPmin-luc2

    Plasmid

    #194383

    Purpose

    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    10xUAS-luc2
    

    Expression

    Mammalian

    Promoter

    MLP minimal

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF12J

    Plasmid

    #42943

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN+ Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only