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pFA6a-5FLAG-hphMX6

Plasmid

#19342

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

hphMX6

Expression

Yeast

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pdCas9-DNMT3A-PuroR (ANV)

Plasmid

#71684

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA4/HisMaxB-YAP1-S127A

Plasmid

#18988

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S127A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 127 changed to Alanine, mutated protein t…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pGL3-TGFb1

Plasmid

#101762

Purpose

TGFb1 promoter reporter

Depositor

Yuh-Shan Jou

Publication

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

transforming growth factor beta 1 promoter (TGFB1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]

Plasmid

#16283

Depositor

Thomas Jessell

Publication

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Insert

HB9 promoter (Mnx1 Mouse)

Use

Mouse Targeting

Tags

IRES-EGFP

Expression

Mammalian

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
LB322 (Bcl-2 from ATG to -3934)

Plasmid

#15381

Depositor

Linda Boxer

Publication

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -3934 with both P1 and…

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3 CB1

Plasmid

#13391

Purpose

Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

Depositor

Mary Abood

Publication

Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

Insert

CB1 (Cnr1 Mouse)

Expression

Mammalian

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Publication

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Publication

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM201

Plasmid

#13078

Depositor

Kenan Murphy

Publication

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Ptac-gam-bet-exo operon

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Publication

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3.1_Lifeact-mCherry

Plasmid

#67302

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

Plasmid

#52053

Purpose

Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.

Depositor

Jesse Rinehart

Insert

sfGFP

Expression

Bacterial

Promoter

tetR/pLtetO

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

elavl3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-cyan pre-eGRASP(p32)

Plasmid

#111579

Purpose

An AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Publication

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCIneo RYR-1

Plasmid

#75113

Purpose

Clone encoding rabbit ryanodine receptor in mammalian expression vector

Depositor

Paul Allen, Robert Dirksen

Publication

Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

Insert

Ryanodine receptor 1

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H2B.1

Plasmid

#42630

Depositor

Joe Landry

Insert

Human H2B.1 (H2BC8 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available since

Feb. 22, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pET His6 MBP N10 TEV LIC cloning vector (2C-T)

Plasmid

#29706

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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