2,293 results

1. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

   Plasmid

   #196224

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK288 (mAID-Bsr)

   Plasmid

   #72826

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Bsr
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-LAMP1-mRFP

   Plasmid

   #169004

   Purpose

   Express rat LAMP1-mRFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

   Insert

   rat LAMP1-mRFP
   

   Use

   Unspecified

   Tags

   mRFP

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mGeos-M-Homer1-C-18

   Plasmid

   #57528

   Purpose

   Localization: Membrane Adaptor, Excitation: 503 / 569, Emission: 514 / 581

   Depositor

   Michael Davidson

   Insert

   Homer1 (Homer1 Rat)
   

   Tags

   mGeos-M

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pORANGE Gria1-GFP KI

   Plasmid

   #131489

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-GLT1b-V5

   Plasmid

   #162518

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1b (Slc1a2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaV1.3e[8a,11,31b,Δ32,42a]

   Plasmid

   #49333

   Purpose

   Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Promoter

   CMV

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-GLT1a-V5

   Plasmid

   #162517

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Green-Camuialpha

    Plasmid

    #26933

    Depositor

    Ryohei Yasuda

    Article

    Lee et al Nature. 2009 Mar 19. 458(7236):299-304.

    Insert

    REACh-CaMKIIa-mEGFP
    

    Tags

    REACh and mEGFP

    Expression

    Mammalian

    Available Since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFsynW KDEL Hook

    Plasmid

    #197277

    Purpose

    Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    Streptavidin
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV mScarlet-LC3B

    Plasmid

    #200431

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    Map1lc3b (Map1lc3b Rat)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-CMV-NES-fRCaMP1

    Plasmid

    #125249

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMp1
    

    Tags

    NES signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNAI-GAL4-CREB

    Plasmid

    #46769

    Purpose

    Expression of GAL4 fusion to wild type CREB

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB (Creb1, GAL4 Rat, Budding Yeast)
    

    Tags

    GAL4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Rlucrarr3-Flash6

    Plasmid

    #74134

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

17. GFP-pcDNA3-PKCgamma-cys1Acys1B

    Plasmid

    #21190

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-cys1Acys1B (Prkcg Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

    Available Since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N3 alpha-SSeCKS

    Plasmid

    #61294

    Purpose

    Mammalian expression of GFP-alpha-SSeCKS

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. AAV Cag Flex H2B Gcamp6f reverse

    Plasmid

    #74155

    Purpose

    Calcium Sensor

    Depositor

    Loren Looger

    Insert

    Gcamp6f
    

    Use

    AAV

    Tags

    H2B

    Promoter

    CAG

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-RUSH-LAMP2A-mNeonGreen

    Plasmid

    #228525

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP2 (Lamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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