2,226 results

1. AAV-hSyn-Axon-jGCaMP8m

   Plasmid

   #172719

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-DNM2-R465W

   Plasmid

   #155262

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 465 to Tryptophan

   Promoter

   Available since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE unc13a GFP KI

   Plasmid

   #131498

   Purpose

   Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Unc13a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-EGFP-PA-BAX(mito)

   Plasmid

   #228942

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK7-HA-S6K1-E389-deltaCT

   Plasmid

   #8993

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T389E, stop codon at amino acid 402

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET42b-HF-BE3

   Plasmid

   #87438

   Purpose

   Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdTomato-KIF1A

   Plasmid

   #102850

   Purpose

   Overexpresses KIF1A and cell fill TdTomato

   Depositor

   Erik Dent

   Article

   McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

   Insert

   KIF1A (Kif1a Rat)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Camui-CR

   Plasmid

   #40256

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Camuiα-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos2-PSD95-N-14

    Plasmid

    #57411

    Purpose

    Localization: Membrane Scaffold, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    PSD95
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUGW-VGAT-pHluorin

    Plasmid

    #207467

    Purpose

    Lentiviral expression of VGAT-pHluorin

    Depositor

    Robert Edwards

    Article

    Jain et al bioRxiv 2023

    Insert

    VGAT (Slc6a12 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCardinal-Lysosomes-20

    Plasmid

    #56166

    Purpose

    Localization: Lysosome Membrane, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    LAMP1
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK294 (mAID-mCherry2-Bsr)

    Plasmid

    #72832

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Use

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    chicken actin

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMXs-puro GFP-b5 ER

    Plasmid

    #38274

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    Cytochrome b5 (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Cyb5a Tranmembrane region from 95 to 134 amino ac…

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

18. HS-aPKC-CAAX-mKate2

    Plasmid

    #105950

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC-CAAX
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid

    #55797

    Purpose

    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFP-NavII-III

    Plasmid

    #26056

    Depositor

    Matthew Grubb

    Article

    Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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