Addgene

Showing: 441 - 460 of 2177 results

Results per page:

Sort results by:

pAAV.GFAP.iGluSnFR3.v857.SGZ

Plasmid

#178337

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP/KATNA1

Plasmid

#89316

Purpose

Expression of rat GFP/Katanin A subunit in mammalian cells

Depositor

Peter Baas

Article

Karabay et al J Neurosci. 2004 Jun 23;24(25):5778-88.

Insert

KATNA1 (Katna1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Article

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Insert

Dynamin 2 K44A (Dnm2 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA TrkC

Plasmid

#24089

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkC (Ntrk3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDHGC5-EGFP

Plasmid

#119933

Purpose

Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-Wnk1

Plasmid

#38779

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-mDlx-jGCaMP8s-WPRE

Plasmid

#176755

Purpose

jGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancer

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-beta-arrestin2

Plasmid

#36919

Depositor

Robert Lefkowitz

Article

Xiao et al J Biol Chem. 2004 Dec 31;279(53):55744-5

Insert

beta-arrestin2 (Arrb2 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only
Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Flex-Axon-GCaMP7b

Plasmid

#135419

Purpose

Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

Depositor

Rylan Larsen

Insert

axon-jGCaMP7b

Use

AAV

Tags

6xHis and GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

Plasmid

#134613

Purpose

Mammalian expression of rat KIF5C 378-955

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5C (Kif5c Rat)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge