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pcDNA3-TLR9-YFP

Plasmid

#13642

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 9 (TLR9 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Use

Tags

SMASh tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Integrin beta 2 - mYFP

Plasmid

#8638

Depositor

Timothy Springer

Article

Kim et al Science 2003 Sep 19;301(5640):1720-5.

Insert

Integrin beta 2 (ITGB2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

stop codon of beta2 was replaced with AgeI restri…

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 5F-L YFP

Plasmid

#84914

Purpose

Mammalian expresion of TDP-43 5F-L YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

F147L, F149L, F194L, F229L, F231L

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBD-EYFP

Plasmid

#13723

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD-EYFP (PAK1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

p21 binding domain of human Pak1(amino acids 65-1…

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Use

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN-YFP-Parkin

Plasmid

#59416

Purpose

to make YFP-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

YFP-Parkin (PRKN Human)

Use

Retroviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3-NCwt-SYFP2

Plasmid

#189765

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-NCwt-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15ABAD- YFP

Plasmid

#186571

Purpose

p15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-YFP

Plasmid

#29592

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TLR10-YFP

Plasmid

#13643

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 10 (TLR10 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Use

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEYFP-Plk1(499)

Plasmid

#39843

Depositor

Jonathon Pines

Article

Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

Insert

Plk1 (PLK1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-SMASh

Use

Tags

SMASh tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ YFP-Smo

Plasmid

#41086

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet3-NCwt-SYFP2

Plasmid

#189766

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-NCwt-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

encodes the expected internal mutations relative …

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-ChR2-YFP

Plasmid

#204345

Purpose

PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

ChR2-YFP

Use

Tags

Yellow fluorescent protein

Expression

Mammalian

Mutation

C-terminus (aa315-737) is deleted - PMID: 1461559…

Promoter

CAG

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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