We narrowed to 6,472 results for: poly

1. pTWIN1-His6-Ssp-Httex1-37Q

   Plasmid

   #84351

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hu-TLR4 299/399 dual snp flag pDEST40

   Plasmid

   #42648

   Purpose

   destination vector for expression of human TLR4 299/399 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 299/399 SNP (TLR4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D299G and T399I

   Promoter

   CMV

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pUDP044

   Plasmid

   #101168

   Purpose

   pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTWIN1-His6-Ssp-Httex1-15Q

   Plasmid

   #84348

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET41s-GST-ATF6a-AD

   Plasmid

   #64774

   Purpose

   bacterial expression of GST-tagged ATF6 (1-150)

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   ATF6a-AD (1-150) (ATF6 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-term deletion

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79889

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-GST-CHD5(Chromos)-FRAG

   Plasmid

   #68875

   Purpose

   IPTG inducible bacterial expression of CHD5 fragment

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only the two chromodomains of CHD5

   Promoter

   tac

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-Flag-Med25-VWA

   Plasmid

   #64772

   Purpose

   Mammalian expression of Flag-tagged Med25-VWA

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VWA (MED25 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C-terminal deletion

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-FLAG-CHD5-WT-331-672aa

   Plasmid

   #68870

   Purpose

   Mammalian expression of CHD5 histone binding domain (331-672aa)

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   331-672aa

   Promoter

   CMV

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. WT USP7 UBL-12

    Plasmid

    #227230

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-WzxE-TEV-His10

    Plasmid

    #226781

    Purpose

    Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

    Depositor

    James Naismith

    Article

    Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

    Insert

    wzxE
    

    Use

    Tags

    TEV cleavable 10xHistidine tag

    Expression

    Bacterial

    Mutation

    valine insertion in position 2 due to the cloning…

    Promoter

    araBAD

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

    Plasmid

    #227103

    Purpose

    DRD2 receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    DRD2-NTEV (DRD2 Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG8Ha-His-EWSR1-359-549

    Plasmid

    #195868

    Purpose

    Expresses the RRM-RG2-ZnF (359-549) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWS 359-549 (EWSR1 Human)
    

    Use

    Tags

    His8-linker-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT-TO-EGFR*-CUbo-GFP

    Plasmid

    #212815

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    8xHis and CUbo-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-EGFR*-CUbo-His6

    Plasmid

    #212819

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

    Plasmid

    #212822

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K63R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

    Plasmid

    #201576

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A, M173A and V195A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)

    Plasmid

    #201460

    Purpose

    Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks N-terminal residues 1-110

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge