We narrowed to 43,334 results for: cha;

1. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA6-LRRC55-V5-Flag-His

   Plasmid

   #127819

   Purpose

   Expression of human LRRC55 in mammalian cells

   Depositor

   Jiusheng Yan

   Article

   Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

   Insert

   LRRC55 (LRRC55 Human)
   

   Use

   Tags

   V5, FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-ATG13

   Plasmid

   #171413

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG13 (ATG13 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NAX_CAGGS_CAS9_m4_KanR neo

   Plasmid

   #167869

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_VPR_Hygro

   Plasmid

   #167883

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEM-hu FceRI beta

   Plasmid

   #8366

   Depositor

   Jean Kinet

   Article

   Kuster et al J Biol Chem 1992 Jun 25;267(18):12782-7.

   Insert

   human Fc epsilon RI beta cDNA (MS4A2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2005

   Availability

   Academic Institutions and Nonprofits only

8. K20‐scFv‐pSMBP2

   Plasmid

   #159940

   Purpose

   To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

   Depositor

   Sandra Schmid

   Article

   Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

   Insert

   anti beta-1 integrin scFv
   

   Use

   Tags

   6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CapChR2_pUAST-attb

   Plasmid

   #188041

   Purpose

   Expresses CapChR2 in Drosophila

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2
   

   Use

   Tags

   none

   Expression

   Insect

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   UAS

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-GfaABC1D-DreO-4x6T

    Plasmid

    #196413

    Purpose

    Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    DreO
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB406

    Plasmid

    #68671

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMpGWB431

    Plasmid

    #68696

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tdEos-VSVG-16

    Plasmid

    #57693

    Purpose

    Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    VSVG
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pTZ tRNA_gRNA empty scaffold

    Plasmid

    #188450

    Purpose

    Expression of gRNAs using a human tRNA promoter

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tRNA glutamine

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OVA-miSFIT-8-G

    Plasmid

    #124675

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pUSE-Src-KD-UniRapR-mCerulean-myc

    Plasmid

    #45382

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-KD-UniRapR-mCerulean-myc
    

    Use

    Tags

    mCerulean and myc

    Expression

    Mammalian

    Mutation

    mSrc R388D (kinase dead), Y529F

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT7-RiboJ-LacZa-T7

    Plasmid

    #173224

    Purpose

    pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. GFP-PIWI

    Plasmid

    #11591

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contain only aa482-869

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

23. NHA-TNGW1

    Plasmid

    #36437

    Depositor

    Edward Chan

    Article

    Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Full length (aa1-1962)

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pSG5-Cyp26a1

    Plasmid

    #13457

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pX458_miR-290-295KO-gRNA1

    Plasmid

    #172709

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX458_miR-290-295KO-gRNA2

    Plasmid

    #172710

    Purpose

    Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLTR PKC beta II

    Plasmid

    #8417

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Rat Protein Kinase C beta II (Prkcb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

28. p-mCherry-N1-iChloC

    Plasmid

    #85467

    Purpose

    improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Insert

    iChloC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, T159C, D156N

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRG/III-hs-MBP-A2aTr316-H10

    Plasmid

    #121683

    Purpose

    Bacterial expression of adenosine A2a receptor fusion protein

    Depositor

    Reinhard Grisshammer, Richard Henderson

    Article

    Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pCDNA6-LRRC38-V5-His

    Plasmid

    #127820

    Purpose

    Expression of human LRRC38 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC38 (LRRC38 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhaR promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NAX_CAGGS_CAS9_m4_VPR_Puro

    Plasmid

    #167887

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. THI VP64 pGAP T-

    Plasmid

    #161487

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMpGWB225

    Plasmid

    #68616

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Triple repeats of Citrine (3xCitrine)

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBS-Squ:mCherry

    Plasmid

    #20163

    Depositor

    Eric Wieschaus

    Article

    Martin et al Nature. 2008 Nov 23. ():.

    Insert

    spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

38. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pT7-RiboJ-mScarlet-T7

    Plasmid

    #173227

    Purpose

    Encodes mScarlet fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    mScarlet
    

    Use

    Cell-free system

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pGMC00014

    Plasmid

    #172526

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Non-targeting guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge