Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 12,055 results for: cel.2

Showing: 11451 - 11500 of 12055 results

  1. pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)

    Plasmid
    #50885
    Purpose
    Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N731C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50885-plasmid-map-sequence-id-76358

    Enlarge

  2. pcDNA3.1 Flag YFP hNKCC1 V673C (NT437)

    Plasmid
    #50874
    Purpose
    Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V673C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50874-plasmid-map-sequence-id-76347

    Enlarge

  3. pcDNA3.1 Flag YFP hNKCC1 I677C (NT467)

    Plasmid
    #50878
    Purpose
    Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I677C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50878-plasmid-map-sequence-id-76351

    Enlarge

  4. pcDNA3.1 Flag YFP hNKCC1 I678C (NT445)

    Plasmid
    #50879
    Purpose
    Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I678C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50879-plasmid-map-sequence-id-76352

    Enlarge

  5. pcDNA3.1 Flag YFP hNKCC1 L736C (NT522)

    Plasmid
    #50890
    Purpose
    Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L736C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50890-plasmid-map-sequence-id-76363

    Enlarge

  6. pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)

    Plasmid
    #50876
    Purpose
    Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50876-plasmid-map-sequence-id-76349

    Enlarge

  7. pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)

    Plasmid
    #50884
    Purpose
    Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50884-plasmid-map-sequence-id-76357

    Enlarge

  8. pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)

    Plasmid
    #50889
    Purpose
    Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50889-plasmid-map-sequence-id-76362

    Enlarge

  9. pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)

    Plasmid
    #50880
    Purpose
    Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S679C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50880-plasmid-map-sequence-id-76353

    Enlarge

  10. pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)

    Plasmid
    #50881
    Purpose
    Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N680C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50881-plasmid-map-sequence-id-76354

    Enlarge

  11. pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)

    Plasmid
    #50864
    Purpose
    Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50864-plasmid-map-sequence-id-76337

    Enlarge

  12. pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)

    Plasmid
    #50869
    Purpose
    Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S679C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50869-plasmid-map-sequence-id-76342

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)

    Plasmid
    #50863
    Purpose
    Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50863-plasmid-map-sequence-id-76336

    Enlarge

  14. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

    Plasmid
    #50868
    Purpose
    Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I677C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50868-plasmid-map-sequence-id-76341

    Enlarge

  15. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

    Enlarge

  16. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR2 (CRHR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213216-plasmid-map-sequence-id-430778

    Enlarge

  18. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

    Enlarge

  19. pcDNA3-β2AR-μDpTT

    Plasmid
    #182326
    Purpose
    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    Use
    Tags
    Full-length human beta2 adrenergic receptor
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182326-plasmid-map-sequence-id-366176

    Enlarge

  20. FLAG-FullLength-TET1-CXXC(24)

    Plasmid
    #124398
    Purpose
    Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Shaun Cowley
    Article
    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.
    Insert
    FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624)
    Promoter
    Available Since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124398-plasmid-map-sequence-id-245258

    Enlarge

  22. FLAG-FullLength-TET1-LLAA(20)

    Plasmid
    #124397
    Purpose
    Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Shaun Cowley
    Article
    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.
    Insert
    FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    L897A/L900A mutations (full length protein is 213…
    Promoter
    Available Since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124397-plasmid-map-sequence-id-245256

    Enlarge

  23. ADRB2-DuET

    Plasmid
    #213181
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213181-plasmid-map-sequence-id-430748

    Enlarge

  29. AGTR2-DuET

    Plasmid
    #213184
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AGTR2 (AGTR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213184-plasmid-map-sequence-id-431748

    Enlarge

  30. HCA2-DuET

    Plasmid
    #213308
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213308-plasmid-map-sequence-id-430846

    Enlarge

  31. FPR2-DuET

    Plasmid
    #213240
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FPR2 (FPR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213240-plasmid-map-sequence-id-430798

    Enlarge

  32. pcDNA3-sPDGFRalpha-Fc (Legacy)

    Plasmid
    #136437
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Pro-217 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136437-plasmid-map-sequence-id-271680

    Enlarge

  43. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid
    #173963
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    Use
    Luciferase
    Tags
    Expression
    Mutation
    1 mutated Coordinator motif
    Promoter
    SV40 promoter
    Available Since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173963-plasmid-map-sequence-id-341768

    Enlarge

  44. pGL3-noSV40-EC1.45-human_min1-opossum_min2

    Plasmid
    #173966
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
    Use
    Luciferase
    Tags
    Expression
    Mutation
    See Depositor Comments Below
    Promoter
    SV40 promoter
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173966-plasmid-map-sequence-id-341757

    Enlarge

  45. pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

    Plasmid
    #173962
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated Coordinator motifs
    Promoter
    SV40 promoter
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173962-plasmid-map-sequence-id-341792

    Enlarge

Showing: 11451 - 11500 of 12055 results