We narrowed to 10,009 results for: ADA

1. pGEMHE-mEGFP-mTrim21ΔRING-Box

   Plasmid

   #105520

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. WTTEM1_pSTC0-zeo

   Plasmid

   #202351

   Purpose

   Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   TEM-1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas4_1_2_VA

   Plasmid

   #186438

   Purpose

   Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-Neo-gp78/JM20

   Plasmid

   #13303

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196085

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196079

   Purpose

   Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-IFT54

    Plasmid

    #218726

    Purpose

    Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

    Insert

    IFT54 (TRAF3IP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCX-SponGee-Kras

    Plasmid

    #134777

    Purpose

    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee-Kras
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA SF-HERC2 F3

    Plasmid

    #55786

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-hPPP3R1

    Plasmid

    #179136

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. px458_2A_GFP_sgRNA_G3BP2_G1

    Plasmid

    #127112

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA G3BP2 (G3BP2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-1-β4 hinge-ear

    Plasmid

    #196692

    Purpose

    Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM4951 (pCV0063)

    Plasmid

    #120084

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHeCAHS-mEGFP-NLS

    Plasmid

    #205014

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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