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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAS9-BFP empty

Plasmid

#80976

Purpose

Cas9, TagBFP, and sgRNA expression

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSC201plus

Plasmid

#164227

Purpose

Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Rhamnose-inducible

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRW398-URA3-BXB1-LP

Plasmid

#235977

Purpose

Plasmids for generation of donor DNA for construction of a BXB1-compatible landing pad p1 with URA3 selection marker.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Insert

URA3

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pRW402-P1int-BXB1

Plasmid

#235979

Purpose

Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Type

Empty backbone

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Km

Plasmid

#46817

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and GAL10

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVA.008

Plasmid

#31809

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

JRTCLCrVA.008

Use

Agrobacterium binary

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Promoter

Acetamidase operone

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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iDuet101a

Plasmid

#17629

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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M3929 his3::KanMX3 Disruptor Converter

Plasmid

#51679

Purpose

Yeast marker swap plasmid for converting HIS3 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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PKC beta I WT

Plasmid

#16378

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCG142

Plasmid

#17246

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 intron:pie-1 promoter in PDONRP4P1R

Use

Gateway Cloning

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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p155 pCMV-EGFP/beta-gal

Plasmid

#8387

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGM34DEST

Plasmid

#74756

Purpose

Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

Gateway cassette

Expression

Worm

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[V-60]

Plasmid

#67013

Purpose

Expresses malE-ELP[V-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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