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Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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Lentiguide-Blast-eNMU710
Plasmid#172658PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locusDepositorInsertpegRNA-eNMUA/pegRNA-eNMUB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-HD252
Plasmid#172656PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD252A/pegRNA-HD252B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
UseTagsExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y96F
Plasmid#47094DepositorInsertAL-103 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y32F
Plasmid#47091DepositorInsertAL-103 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL Q42K C214S
Plasmid#47089DepositorInsertAL-09 FL Q42K C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationQ42K C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL I34N C214S
Plasmid#47088DepositorInsertAL-09 FL I34N C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationI34N C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y96F
Plasmid#47085DepositorInsertAL-09 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y91F
Plasmid#47084DepositorInsertAL-09 Y91F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY91FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F Y96F
Plasmid#47083DepositorInsertAL-09 Y32F Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32F Y96FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F
Plasmid#47082DepositorInsertAL-09 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S in pAR9 (rep/cap)
Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only