We narrowed to 7,350 results for: Lif;

1. pDONR221-PARP15

   Plasmid

   #184471

   Purpose

   For gateway cloning of PARP15

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETA-qmTQ2-ATP

   Plasmid

   #225247

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETA-qmTQ2-glucose

   Plasmid

   #225252

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-glucose
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETA-qmTQ2-cAMP

   Plasmid

   #225249

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-SFFV_NKX2-1_Short_V5-GFP

   Plasmid

   #221500

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-EF1a_NKX2-1_CdTag-HA

   Plasmid

   #221493

   Purpose

   NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_NKX2-1_DelCTerm_1-223AA

   Plasmid

   #221476

   Purpose

   Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Deletion of 224-371AA C-terminal domain

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_NKX2-1_DelNTerm_160-371AA

   Plasmid

   #221475

   Purpose

   Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Deletion of 1-159AA N-terminal domain

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_NKX2-1_HD_160-223AA

   Plasmid

   #221477

   Purpose

   Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Deletion of 1-159AA N-terminal and 224-371AA C-te…

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221487

    Purpose

    NKX2-1 N-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-hPGK_NKX2-1_Short_V5-GFP

    Plasmid

    #221498

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX-EF1a_NKX2-1_DelCTerm_1-223AA

    Plasmid

    #221488

    Purpose

    NKX2-1 C-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 224-371AA C-terminal domain

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPI161

    Plasmid

    #207439

    Purpose

    Shuttle vector LifeAct-mCherry

    Depositor

    Rob Kay

    Article

    Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

    Insert

    LifeAct-mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-dErbB4

    Plasmid

    #197357

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPRv2-bleo-ErbB4

    Plasmid

    #197353

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-dErbB1

    Plasmid

    #197354

    Purpose

    A knock-out vector for dog ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-dErbB2

    Plasmid

    #197355

    Purpose

    A knock-out vector for dog ErbB2.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-dErbB3

    Plasmid

    #197356

    Purpose

    A knock-out vector for dog ErbB3.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.539

    Plasmid

    #184958

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fabH donor G954A

    Promoter

    T7/lac

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFR-mScarletI-antiFRET-P2A-EGFP

    Plasmid

    #191458

    Purpose

    Compare the intensity of EGFP to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    EGFP
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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