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Showing: 4581 - 4600 of 9959 results

  1. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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  2. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Publication
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  3. pBABE-ProDOL

    Plasmid
    #206866
    Purpose
    Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag
    Depositor
    Dirk-Peter Herten
    Publication
    Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d
    Insert
    ProDOL (Lyn Human, Aequorea victoria, Rhodococcus rhodochrous, Mouse)
    Use
    Retroviral
    Tags
    HaloTag, His-Tag, SNAP-tag, and eGFP
    Expression
    Mammalian
    Mutation
    Contains Lyn amino acids 1-16
    Available since
    Aug. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    206866-plasmid-map-sequence-id-418214

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  4. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid
    #171813
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171813-plasmid-map-sequence-id-338044

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  5. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171801-plasmid-map-sequence-id-337972

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  6. Venus-Bad-4E-pEGFP-C1

    Plasmid
    #166740
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Publication
    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1
    Insert
    Bad-4E (BAD Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166740-plasmid-map-sequence-id-329000

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  7. pcDNA3.2-Cx43-ML-HA

    Plasmid
    #49857
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
    Depositor
    Robin Shaw
    Publication
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. Cont…
    Promoter
    CMV
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

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  10. NST3_pOpOn2.1

    Plasmid
    #228066
    Purpose
    Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Publication
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3
    Available since
    Jan. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228066-plasmid-map-sequence-id-456796

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  14. GEST-COMP-blac-flag-his

    Plasmid
    #111015
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    gamete egress and sporozoite traversal protein (GEST) (PF3D7_1449000 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111015-plasmid-map-sequence-id-224578

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  15. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  16. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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Showing: 4581 - 4600 of 9959 results