We narrowed to 18,470 results for: Met

1. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

   Plasmid

   #55649

   Purpose

   Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre off/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Short Ef1

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

   Plasmid

   #55646

   Purpose

   Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp off chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTetON-DeAct-GS1

   Plasmid

   #89454

   Purpose

   Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOTTC337 - pAAV EF1a DIO mCherry

   Plasmid

   #47636

   Purpose

   An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pclbw-opa1(isoform 7DeltaS1)-myc

   Plasmid

   #62846

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Promoter

   cmv-bactin

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MSCV p2GM AMPK alpha2hp1 alpha1hp1

   Plasmid

   #89492

   Purpose

   shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   2 hairpins against mouse/human AMPKalpha1 and alpha2
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   U6 (The GFP-Puro is from a PGK promoter; the shRN…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CAG-Chronos-GFP

   Plasmid

   #99232

   Purpose

   AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pOTTC364 - pAAV CaMKIIa iRFP

    Plasmid

    #47903

    Purpose

    An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFA344C7

    Plasmid

    #49236

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TALE-TET1CD Backbone (TET1 Synthetic, Human)
    

    Use

    TALEN; Tale-tet1 expression vector

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Short Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CaMKII-Chrimson-GFP

    Plasmid

    #62718

    Purpose

    AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mIFP12-Fibrillarin-7

    Plasmid

    #56252

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid

    #85072

    Purpose

    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. InsRA-TagBFP

    Plasmid

    #79791

    Purpose

    Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Tags

    TagBFP extracellular

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. mIFP-MAPTau-N-10

    Plasmid

    #56230

    Purpose

    Localization: Microtubules, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    MAPTau (MAPT Human)
    

    Tags

    mIFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid

    #176534

    Purpose

    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associated IscB (Delaware Bay metagenome)
    

    Tags

    14xHis-MBP-bdSUMO and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α1.1-tdTomato

    Plasmid

    #122101

    Purpose

    AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Promoter

    EF1a（1.1kb short version)

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLINC-AKAR1-CAAX

    Plasmid

    #87705

    Purpose

    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FLINC-AKAR1-CAAX
    

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge