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Showing: 4601 - 4620 of 41347 results

  1. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  5. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

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  12. Venus2-Y705F/S727A-STAT3

    Plasmid
    #123177
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123177-plasmid-map-sequence-id-238660

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  16. Venus1-Y705F/S727A-STAT3

    Plasmid
    #123176
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123176-plasmid-map-sequence-id-238648

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  17. SARS CoV-2 RBD (bio-His)

    Plasmid
    #166857
    Purpose
    Human expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.
    Depositor
    Evelien Bunnik
    Article
    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d
    Insert
    SARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
    Tags
    6xHis and Biotinylation site
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166857-plasmid-map-sequence-id-327669

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Showing: 4601 - 4620 of 41347 results