We narrowed to 30,231 results for: REP

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pMT2

Plasmid

#206062

Purpose

expression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-IEo

Plasmid

#201992

Purpose

AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Depositor

Wei Xu

Article

Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

Insert

Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Use

AAV

Promoter

Tight TRE

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A

Promoter

MESV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-LC3B

Plasmid

#200427

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10ΔF3

Plasmid

#194857

Purpose

Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF3 (MYO10 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncated MYO10 construct. MYO10 amino acids 1-1…

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TERTp124_Gluc_CCR5

Plasmid

#192271

Purpose

Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-124 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp146_Gluc_CCR5

Plasmid

#192272

Purpose

Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-146 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7

Plasmid

#186753

Purpose

A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Type

Empty backbone

Use

Synthetic Biology; Cloning backbone

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

Plasmid

#184636

Purpose

Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Mutation

Gat1 gene with Arginine 69 changed to Lysine, to …

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT2h70CRET(#325)

Plasmid

#184060

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Hsp70-4

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2G2A

Plasmid

#174334

Purpose

encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

mutation of a.a 2 Gly to Ala

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2EFMUT

Plasmid

#174333

Purpose

encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

NKD2132DFD134>AFA (substitutions of D132 and D…

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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