We narrowed to 5,649 results for: pCas

1. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiV_Cas9_Blast

   Plasmid

   #125592

   Purpose

   Lentiviral expression of spCas9 with blasticidin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e(TadA-8e V106W)

   Plasmid

   #138495

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V106W)-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e V106W

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH6HTC_PRDX6-Halo

   Plasmid

   #175338

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCutamp

   Plasmid

   #140632

   Purpose

   Plasmid-curing in Escherichia coli by targeting the AmpR promoter

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-TSF-TEVcs-ULK1

   Plasmid

   #171416

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ULK1 (ULK1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Puro-Cas9 donor

   Plasmid

   #58409

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrichoGate-1

    Plasmid

    #160701

    Purpose

    derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LacZ bordered by BsaI restrction sites

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMPE-ePPEplus

    Plasmid

    #205242

    Purpose

    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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