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Showing: 4621 - 4640 of 160950 results

  2. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  6. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222080-plasmid-map-sequence-id-438352

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  7. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid
    #73147
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-NRX
    Use
    Lentiviral
    Tags
    2x HA tag and Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73147-plasmid-map-sequence-id-134524

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  11. pCXLE-SOX2-17-p2a-KLF4

    Plasmid
    #193290
    Purpose
    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193290-plasmid-map-sequence-id-379665

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  13. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  14. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Use
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  19. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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Showing: 4621 - 4640 of 160950 results