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Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRK-flag-USP19 494-1216 (USP)
Plasmid#78588PurposeExpression flag-USP19 494-1216 (USP) in mammalian cellsDepositorInsertflag-USP19 494-1216 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493 and mutated 1216 residue…PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTV-mHdac1-tdiRFP670-PEST2
Plasmid#122351PurposeTargeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorInsert2A-tdiRFP-PEST with homology arms for Hdac1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159848PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159856PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RIPK3 4KR
Plasmid#78821PurposeTo overexpress RIPK3 4KR in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationK55R, K89R, K363R, K501RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR1 MutSBE
Plasmid#15718DepositorInsertp15 4xSBR1 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant SBE (Smad binding element)PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTV-Met-Eif4a1-tdiRFP670-PEST
Plasmid#122371PurposeTargeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.DepositorInsert2A-tdiRFP-PEST-2A with homology arms for Eif4a1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Eif4a1-L
Plasmid#122346PurposeExpresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Eif4a1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RIPK1 K115R
Plasmid#78843PurposeTo overexpress RIPK1 K115R in Mammalian CellsDepositorInsertRIPK1 (RIPK1 Human)
UseTagsFLAGExpressionMammalianMutationK115RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc- mCE(Del 25C)
Plasmid#82472PurposeExpresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminalDepositorInsertmyc-mCE-C terminal deleted (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
NHA-PAZ(aa1-480)
Plasmid#21530DepositorInsertAgo2 (AGO2 Human)
UseTagsNHAExpressionMammalianMutationcontain only aa1-480PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_RNF138_G1
Plasmid#127120DepositorInsertgRNA RNF138 (RNF138 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71345PurposeExpress Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInserthOTC_Cyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357E
Plasmid#18883DepositorInsertYap1 Y357E (YAP1 Human)
UseTagsFlagExpressionMammalianMutationY357EPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMflST-o4
Plasmid#101316PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(S431A)
Plasmid#84641PurposemCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation siteDepositorInsertmCherry-LBK1(S431A) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPKA phosphorylation site Serine 428 mutated to Al…PromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only