We narrowed to 13,263 results for: cDNA

1. pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

   Plasmid

   #164018

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Y527A

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

   Plasmid

   #162639

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

   Plasmid

   #163989

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W1460A

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

   Plasmid

   #163987

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y1445A

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

   Plasmid

   #163986

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1427E

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

   Plasmid

   #163985

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1414R

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

   Plasmid

   #163984

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1411R

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

   Plasmid

   #163983

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1401R

   Promoter

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

   Plasmid

   #164132

   Purpose

   Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   ciCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

    Plasmid

    #163982

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1397R

    Promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

    Plasmid

    #163981

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R1392E

    Promoter

    Available Since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E

    Plasmid

    #163980

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R1386E

    Promoter

    Available Since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid

    #131255

    Purpose

    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-GFP-EBA175-Flag(M1423R/P1424V)

    Plasmid

    #155309

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    M1423R/P1424V; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-GFP-EBA175-Flag(P1424V/Y1425W)

    Plasmid

    #155310

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424V/Y1425W; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-GFP-EBA175-Flag(P1424V/Y1426H)

    Plasmid

    #155311

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424V/Y1426H; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-GFP-EBA175-Flag(P1424Q/Y1426S)

    Plasmid

    #155003

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424Q/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-GFP-EBA175-Flag(P1424R/Y1426S)

    Plasmid

    #155004

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424R/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-GFP-EBA175-Flag(P1424D/Y1426S)

    Plasmid

    #155005

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424D/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid

    #72889

    Purpose

    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Inserts

    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127317

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

    Plasmid

    #127409

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, E309C …

    Promoter

    CMV

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

    Plasmid

    #127406

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

    Plasmid

    #127402

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

    Plasmid

    #127400

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127325

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127320

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127319

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127324

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

    Plasmid

    #127329

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge