We narrowed to 11,687 results for: NSI

1. pOAD

   Plasmid

   #196825

   Purpose

   Yeast two-hybrid ; Gal4 activation domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Tags

   Gal4 Activation domain

   Expression

   Yeast

   Promoter

   ADH

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. wt1aCreERT2

   Plasmid

   #108464

   Purpose

   Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   wt1aCreERT2
   

   Use

   Cre/Lox

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. human b1 ecto-pIRES

   Plasmid

   #113387

   Purpose

   Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

   Insert

   human beta 1 ectodomain
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ-432

   Plasmid

   #195463

   Purpose

   Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - SMASh - ZF3 - p65 - ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ-431

   Plasmid

   #195462

   Purpose

   Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - ZF3 - p65 - ERT2 - SMASh
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiGuide_GFP_sgEPAS1#1

   Plasmid

   #183119

   Purpose

   pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiGuide_GFP_sgEPAS1#2

   Plasmid

   #183120

   Purpose

   pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EPAS1-2
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-roxSTOProx-LynEGFP

   Plasmid

   #133923

   Purpose

   Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   LynEGFP
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLC-mEos2

   Plasmid

   #38010

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Tags

   mEos2

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB9337 (pgRNA_IV-1_NatMX)

    Plasmid

    #161590

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX6p1 AO7RE

    Plasmid

    #137843

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2 GFP-GSK3-MAPK-E3mut

    Plasmid

    #29691

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site mutated

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psiCHECK-2-Zfp36-3'UTR

    Plasmid

    #61780

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR
    

    Use

    Luciferase

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Prohibitin-bio-His

    Plasmid

    #50800

    Purpose

    Expresses enzymatically monobiotinylated full length Prohibitin

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    Prohibitin
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Expression

    Mammalian and Yeast

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

    Plasmid

    #191151

    Purpose

    Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L72:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

    Plasmid

    #194934

    Purpose

    LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-puro-mTurquoise2-Nuf2

    Plasmid

    #80761

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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