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We narrowed to 32,758 results for: FER

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Showing: 4641 - 4660 of 32758 results

  1. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  7. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  11. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  12. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  13. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  16. pGL3-NBRE3

    Plasmid
    #208254
    Purpose
    pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells
    Depositor
    Douglas Kojetin
    Article
    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156
    Insert
    3X NBRE-LUC
    Expression
    Mammalian
    Promoter
    SV40 early promoter
    Available Since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    208254-plasmid-map-sequence-id-417965

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Showing: 4641 - 4660 of 32758 results