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pQCXIZ GFP PODXL delta PBM + Ezrin

Plasmid

#202418

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

Ezrin and GFP

Mutation

delete PDZ binding motif

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro2-luc

Plasmid

#101798

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET1-DHDDS/Sumo-NgBR

Plasmid

#203162

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

Inserts

DHDDS (DHDDS Human)
NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

truncation

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro3-luc

Plasmid

#101799

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-BRAF-REG-Halo

Plasmid

#194771

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV51

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTNG4kwh

Plasmid

#44722

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS::EGFP

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-GLI1-P2A-mCherry

Plasmid

#203858

Purpose

Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

GLI1 (GLI1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

Plasmid

#172637

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GALT5catdead-blast

Plasmid

#220869

Purpose

lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

beta-1,3-galactosyltransferase 5 (B3GALT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

encodes D156A and D243A mutations; also, nucleoti…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

Plasmid

#69114

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

L1939A and L1942A amino acid substitutions. Sile…

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-MAFB-P2A-mCherry

Plasmid

#203866

Purpose

Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

MAFB (MAFB Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-pGK-nAChRb2(E61C)-IRES-GFP

Plasmid

#164777

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

E61C

Promoter

pGK

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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px458-CCND1

Plasmid

#172630

Purpose

Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against CCND1 (CCND1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

Plasmid

#172647

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2-Puro

Plasmid

#172626

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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