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pET - FnR062.Y78V

Plasmid

#84801

Purpose

bacterial expression of FnR.0.6.2-Y78V protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78V

Tags

6xHis

Expression

Bacterial

Mutation

Y78V

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78A

Plasmid

#84784

Purpose

bacterial expression of FnR.0.6.2-Y78A protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78A

Tags

6xHis

Expression

Bacterial

Mutation

Y78A

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78C

Plasmid

#84785

Purpose

bacterial expression of FnR.0.6.2-Y78C protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78C

Tags

6xHis

Expression

Bacterial

Mutation

Y78C

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78E

Plasmid

#84787

Purpose

bacterial expression of FnR.0.6.2-Y78E protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78E

Tags

6xHis

Expression

Bacterial

Mutation

Y78E

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78L

Plasmid

#84793

Purpose

bacterial expression of FnR.0.6.2-Y78L protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78L

Tags

6xHis

Expression

Bacterial

Mutation

Y78L

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78M

Plasmid

#84794

Purpose

bacterial expression of FnR.0.6.2-Y78M protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78M

Tags

6xHis

Expression

Bacterial

Mutation

Y78M

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78N

Plasmid

#84795

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Expression

Bacterial

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78Q

Plasmid

#84797

Purpose

bacterial expression of FnR.0.6.2-Y78Q protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78Q

Tags

6xHis

Expression

Bacterial

Mutation

Y78Q

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78T

Plasmid

#84800

Purpose

bacterial expression of FnR.0.6.2-Y78T protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78T

Tags

6xHis

Expression

Bacterial

Mutation

Y78T

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pSB1C3-J23110-B0034-mRFP1

Plasmid

#160448

Purpose

BioBrick mammalian-codon-optimized mRFP1

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-dsRed

Plasmid

#160449

Purpose

BioBrick mammalian-codon-optimized dsRed

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, dsRed

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCh-cpFRB1

Plasmid

#155389

Purpose

Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

cpFRB1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-cpFRB2

Plasmid

#159645

Purpose

Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

cpFRB2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

Plasmid

#115429

Purpose

Selection for in-frame fusion expression constructs

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Type

Empty backbone

Tags

T2A-SpHIS5 and λN

Expression

Yeast

Promoter

PGK1

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pKER-Clover

Plasmid

#85974

Purpose

Expresses Clover from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

Clover

Expression

Mammalian

Promoter

Ef-1a

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pWW2149

Plasmid

#80392

Purpose

Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Insert

Taz Histidine Kinase

Use

Synthetic Biology

Tags

Fused by GS linkers to 4 SH3 domains

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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