We narrowed to 18,341 results for: met

1. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

   Plasmid

   #47904

   Purpose

   An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   eNpHR3.0
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST CDC42EP1 S192A

   Plasmid

   #69820

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S192A

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CBh-Alb-mNG

   Plasmid

   #183466

   Purpose

   To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-CIB1-mCerulean-MP

   Plasmid

   #58366

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC374 - pAAV EF1a DIO iRFP

   Plasmid

   #47626

   Purpose

   An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEN6-Ubc5 Sc

   Plasmid

   #83234

   Purpose

   pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Inserts

   Ubiqutin (UBC Human)
   Ubc 5 (UBC5 Budding Yeast)
   Uba 1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

   Plasmid

   #55645

   Purpose

   Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV5, AAV8, and AAV9

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mouse Ism1 with C-terminal Myc-6X-his tag

    Plasmid

    #173046

    Purpose

    Expresses Ism1 in mammalian cells

    Depositor

    Katrin Svensson

    Article

    Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

    Insert

    Ism1 (Ism1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-MEKDD

    Plasmid

    #31202

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    MAPKK1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S218D S222D

    Promoter

    Available Since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid

    #89463

    Purpose

    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    E. coli DHFRdd destabilization domain and mCherry…

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCardinal-Farnesyl-5

    Plasmid

    #56159

    Purpose

    Localization: Plasma Membrane, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. LMP AMPK alpha1 hp1

    Plasmid

    #89489

    Purpose

    shRNA against AMPK alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-CIB1-mRFP1-MP

    Plasmid

    #58367

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

    Plasmid

    #178707

    Purpose

    AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    Dreadd-hM3Dq-HA-P2A-tBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid

    #193051

    Purpose

    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    ALA7-TMD
    

    Use

    Tags

    Ig k-chain leader sequence, MBP, and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-H-RAS V12

    Plasmid

    #31201

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    H-Ras (HRAS Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G12V constitutively active

    Promoter

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only