We narrowed to 165,307 results for: addgene

1. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-CAG-Sun1-GFP-WPRE-pA

   Plasmid

   #220752

   Purpose

   Expression of Sun1GFP for nuclei isolation

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   Sun1-GFP-WPRE
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-KRAB-dCas9-IRES-GFP

   Plasmid

   #85556

   Purpose

   2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMW948: CAG-DmrC-NLS-3xFLAG-VPR

   Plasmid

   #104565

   Purpose

   Mammalian expression vector for DmrC fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   DmrC-VPR
   

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Expression

   Worm

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDYH5

   Plasmid

   #52032

   Purpose

   Expression of GFP-tagged DAF-16 in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   DAF-16 (daf-16 Nematode)
   

   Tags

   GFP and HIS

   Expression

   Worm

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. KRAB-dSpCas9

    Plasmid

    #205248

    Purpose

    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170810

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_TT 3xFLAG-TWIST2

    Plasmid

    #197935

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTKM07

    Plasmid

    #233479

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol1-UAS:ChrimsonR-tdTomato

    Plasmid

    #124231

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChrimsonR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV, lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only