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SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ytdTomato-URA3

Plasmid

#168054

Purpose

Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Tags

ytdTomato

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-URA3

Plasmid

#168059

Purpose

Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCKmatBC

Plasmid

#138587

Purpose

matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)

Use

Synthetic Biology

Expression

Bacterial

Mutation

matB contains native GUG start codon

Promoter

Plac promoter

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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miReporter-PGK

Plasmid

#82477

Purpose

microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Expression

Bacterial

Mutation

NA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDV10_pCMV-ADAR1-p150

Plasmid

#200405

Purpose

Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p150

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDV09_pCMV-ADAR1-p110

Plasmid

#200404

Purpose

Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p110

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRVL-2

Plasmid

#104580

Purpose

Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP1_r1c3

Plasmid

#65724

Purpose

Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-RT001-LVB

Plasmid

#163381

Purpose

Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG17-VEEGFRtm-BFP (LVB)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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