We narrowed to 18,370 results for: Tec

1. pCfB6682

   Plasmid

   #106152

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TAL2236

   Plasmid

   #36687

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD4-TALEN_Left (Chd4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pEU3-NII-HaloLICNot

   Plasmid

   #140185

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   Halo

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

   Plasmid

   #171431

   Purpose

   Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   hH2A-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ

   Plasmid

   #192427

   Purpose

   To act as the on state for testing of the alt Vika recombinase.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::vox-OCS-vox::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

   Plasmid

   #192432

   Purpose

   To act as a 2-input state of the NOR gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-B3RT-Rluc-B3RT-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 SV40-AcrIIA4 (GB3333)

   Plasmid

   #160605

   Purpose

   SV40-AcrIIA4 codon optimized for N.benthamiana

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LC3-mEYFP

   Plasmid

   #182865

   Purpose

   Autophagy Marker

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

   Insert

   rat LC3 (Anxa3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-zeo-2xStrep-BFLF1

   Plasmid

   #162661

   Purpose

   Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   BFLF1
   

   Use

   Lentiviral

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. P16ΔS:S10-(MCS)-3xHA

    Plasmid

    #108183

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM545-Tier1-PhCMV-Citrine

    Plasmid

    #169505

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven yellow fluorescent protein citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACBB-TetT-LVA

    Plasmid

    #32555

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    LVA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. PB-HD118

    Plasmid

    #172655

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD118A/pegRNA-HD118B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPU-Empty-miR30-L1221

    Plasmid

    #132940

    Purpose

    All-in-one control rescue-control shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPU-huTRIMmiR30-L1221

    Plasmid

    #132937

    Purpose

    All-in-one huTRIM5 rescue-control shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH41-Tier1-PhCMV-GLuc

    Plasmid

    #169544

    Purpose

    Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Gaussia Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-TAPP1-PH

    Plasmid

    #67675

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of TAPP1-PH

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    TAPP1-PH
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEAST2-sfGFP

    Plasmid

    #126575

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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