164,577 results

1. pTmpl-miniDonor-GFP

   Plasmid

   #238107

   Purpose

   Mini donor expressing GFP, used for PCR to generate IVT template.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE2-P2A-GFP

   Plasmid

   #132776

   Purpose

   Prime editing in mammalian cells with co-translational GFP expression

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2-P2A-GFP
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #195488

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLifeAct-miRFP670nano

   Plasmid

   #127434

   Purpose

   F-Actin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LifeAct-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-IL13-CD8aStalk-PDGFR

   Plasmid

   #240242

   Purpose

   Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   IL-13 (IL13 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHaloTag-EGFP

   Plasmid

   #86629

   Purpose

   Expresses HaloTag-eGFP tandem (cytosolic)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZsGreen1-N1

   Plasmid

   #54702

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   ZsGreen1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Use

    Tags

    Met-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. JD150

    Plasmid

    #229485

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, E4orf6, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS_L_EBOV

    Plasmid

    #103052

    Purpose

    Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-mDyn3 pcDNA3

    Plasmid

    #36265

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 3 (Dnm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Promoter

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TadCBE-Cas9NG

    Plasmid

    #225093

    Purpose

    Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

    Insert

    TadCBE-Cas9NG
    

    Use

    CRISPR

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TDP-43 (NLSmut)

    Plasmid

    #235489

    Purpose

    Expression of human TDP-43-EGFP with mutated nuclear localization sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, K95A, K97A, R98A

    Promoter

    Available Since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIV151

    Plasmid

    #226180

    Purpose

    Expresses Cas9 in a backbone compatible with K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAP1

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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