We narrowed to 62,913 results for: cloning

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Publication

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCXN2KS-Dsup

Plasmid

#90019

Purpose

To establish stable line expressing Dsup in constitutive manner

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Expression

Mammalian

Promoter

CAG

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_IDH1_p.R132C

Plasmid

#81726

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

R132C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_AXIN2_WT

Plasmid

#82099

Purpose

Gateway Donor vector containing AXIN2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AXIN2 (AXIN2 Human)

Use

Gateway Cloning

Mutation

P50S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Publication

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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GST-thr-RAF1-RBD

Plasmid

#86033

Purpose

Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

Depositor

Dominic Esposito

Insert

RAF1 RBD (Ras-binding domain) (RAF1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains residues 1-149

Promoter

T7

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p5E-krt5

Plasmid

#82585

Purpose

5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

Depositor

Kryn Stankunas

Publication

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

zebrafish krt5 promoter (krt5 Zebrafish)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDECKO-mCherry GFP

Plasmid

#78535

Purpose

Control plasmid (paired gRNAs against GFP)

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Publication

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCLX-CHOP-dGFP

Plasmid

#71299

Purpose

Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

Depositor

Patrick Salmon

Publication

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Promoter

CHOP (ATF-responsive)

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Publication

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway Cloning

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJZC48

Plasmid

#62336

Purpose

sgRNA + 1x COM with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDest eGFP CUGBP1

Plasmid

#61276

Purpose

Mammalian expression of EGFP-tagged human CUGBP1

Depositor

Nicolas Charlet-Berguerand

Insert

CUG-BP (CELF1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU3-gRNA

Plasmid

#53063

Purpose

Transcription of guide RNA (gRNA) in rice cells

Depositor

Caixia Gao

Publication

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

OsU3 promoter + gRNA scaffold

Use

CRISPR; Plant expression

Promoter

rice U3

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pThy1-Flpbow1

Plasmid

#45180

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Flpbow1

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt9A-V5

Plasmid

#43820

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT9A (WNT9A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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