We narrowed to 143,164 results for: ins

1. MSCV-6NBC-GFP-PGK-Puro-Barcode13

   Plasmid

   #83690

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode13
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-6NBC-GFP-PGK-Puro-Barcode14

   Plasmid

   #83691

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode14
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK560

   Plasmid

   #71585

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed arginine-232 to histidine

   Promoter

   T7 promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK504

   Plasmid

   #71584

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-357 to tyrosine

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L284F Cus2p

   Plasmid

   #73059

   Purpose

   S. cerevisiae Cus2p gene with L284F Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Leucine 284 to Phenylalanine

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. D282N Cus2p

   Plasmid

   #73060

   Purpose

   S. cerevisiae Cus2p gene with D282N Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Asp 282 to Asn

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. WT Ecm2

   Plasmid

   #169922

   Purpose

   Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   Ecm2
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNLF1-C_PLCG1:1-1291

    Plasmid

    #211089

    Purpose

    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PLCG1:M1-L1291 (PLCG1 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    isoform 2. I813T natural variant (VAR_011908)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBiT3.1-N_FBXW7:1-707

    Plasmid

    #211099

    Purpose

    Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBiT3.1-C_FBXW7:1-707

    Plasmid

    #211104

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNLF1-N_CCT5:1-541

    Plasmid

    #210978

    Purpose

    Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNLF1-N_FBXW7:1-707

    Plasmid

    #210946

    Purpose

    Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNLF1-C_FBXW7:1-707

    Plasmid

    #210951

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBiT3.1-N_LRRK2:2142-2527

    Plasmid

    #211112

    Purpose

    Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    LRRK2:R2142-E2527 (LRRK2 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    M2397T natural variant (VAR_024965)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBiT3.1-N_DDB1:1-1140

    Plasmid

    #211147

    Purpose

    Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140 (DDB1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    loop deletion I396-D689

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBiT3.1-N_EIF3B:186-685

    Plasmid

    #211205

    Purpose

    Mammalian expression of human EIF3B fragment V186 to T685. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    EIF3B:V186-T685 (EIF3B Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBiT3.1-C_DDB1:1-1140

    Plasmid

    #211188

    Purpose

    Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140 (DDB1 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    loop deletion I396-D689

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBiT3.1-C_LRRK2:2142-2527

    Plasmid

    #211151

    Purpose

    Mammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    LRRK2:R2142-E2527 (LRRK2 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    M2397T natural variant ( VAR_024965)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge