We narrowed to 18,681 results for: tec

1. pBJ6

   Plasmid

   #167135

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Kanamycin promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmKalama1-Nuc

   Plasmid

   #14894

   Purpose

   Mammalian expression of mKalama1 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pmKalama1-Nuc
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   I178T mismatch, replace EYFP of the vector pEYFP-…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. A3A-Gam

   Plasmid

   #119769

   Purpose

   Conversion of C to T monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b LFn-Fla

   Plasmid

   #84871

   Purpose

   Bacterial expression of LFn-Fla fusion

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSplit-CO-hDib1

   Plasmid

   #58788

   Purpose

   Express LucC-hDib1 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDib1 (TXNL4A Human)
   

   Use

   Tags

   Flag and Luciferase (394-550aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CP1041-CBEmax

   Plasmid

   #119803

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1041) -UGI-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcCAST-sgRNA_entry (CJT83)

   Plasmid

   #181785

   Purpose

   Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

   Plasmid

   #191139

   Purpose

   Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   t17d9b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

   Plasmid

   #50022

   Purpose

   expresses Membrane tagged G-CaMP3 in cells expressing Cre

   Depositor

   David Anderson

   Article

   Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

   Insert

   G-CaMP3
   

   Use

   AAV

   Tags

   MARCKS sequence (MGCCFSKT)

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTNSQCas

    Plasmid

    #219434

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonor-LoxP

    Plasmid

    #219436

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    two transposon ends, LoxP-terminator-LoxP gene cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT44c

    Plasmid

    #138511

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9-UGI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GWF058

    Plasmid

    #133609

    Purpose

    UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    Gal4DBD-NS3a, DNCR2-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CP1249-ABEmax

    Plasmid

    #119809

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBJ5

    Plasmid

    #167134

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH607-Tier1-PCREm4-Nluc

    Plasmid

    #169594

    Purpose

    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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