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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG_OGT

Plasmid

#155196

Purpose

N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

lacking N-terminal Met

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_mEGFP-SNAP-tag2

Plasmid

#226513

Purpose

Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

mEGFP-SNAP-tag2

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-PCYT2

Plasmid

#81074

Purpose

Expresses PCYT2 in mammalian cells

Depositor

Erin O'Shea

Article

Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

Insert

phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Malibu-mScarlet

Plasmid

#229554

Purpose

Fluorescent reporter for malonyl-CoA

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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mEmerald-Rad21-C-18

Plasmid

#54247

Purpose

Localization: Centromere, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Rad21 (RAD21 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pT2A-EKAREV-NLS

Plasmid

#173856

Purpose

Encoding EKAREV-NLS.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

EKAREV-NLS

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP

Plasmid

#85426

Purpose

Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-2

Use

Lentiviral

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDRM166 LKB (Luciferase mKate2 Blast)

Plasmid

#183502

Purpose

Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
mKate2
BSD

Use

Lentiviral and Luciferase

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available Since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

Plasmid

#236249

Purpose

Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-CuO

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLGB30

Plasmid

#126620

Purpose

Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE31

Plasmid

#90373

Purpose

Hippo - TEAD1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

Hippo

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGR595

Plasmid

#213785

Purpose

Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

Depositor

Chang Liu

Insert

BadBoy2 TP-DNAP1

Use

Synthetic Biology

Expression

Yeast

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-miR-124-sponge_rev(neg. CTRL)

Plasmid

#117326

Purpose

Dual luciferase assay negative control for miR-124 binding

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

reverse miR-124 sponge

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HIPK1-myc-his

Plasmid

#1850

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

HIPK1 (Hipk1 Mouse)

Tags

his and myc

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only