We narrowed to 34,448 results for: CaS;

1. pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

   Plasmid

   #178998

   Purpose

   Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

   Plasmid

   #178997

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166134

   Purpose

   This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

   Plasmid

   #91802

   Purpose

   Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126756

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121815

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121820

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124283

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

14. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124285

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

15. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124284

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

    Plasmid

    #113696

    Purpose

    U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

    Plasmid

    #109315

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    Mttp gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

    Plasmid

    #113695

    Purpose

    U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

    Plasmid

    #101206

    Purpose

    Bacterial expression plasmid for SpCas9 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid

    #101192

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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