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Plasmid#167652PurposeExpress colicinB-His6, M261CDepositorInsertcba-his6 M261C
UseTagsExpressionBacterialMutationcba-his6 with M261C mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB237.1
Plasmid#170031PurposeEncodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235.2
Plasmid#170025PurposeEncodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB237.2
Plasmid#170032PurposeEncodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
C122-E15: mOrange
Plasmid#162895PurposeStandard Gateway entry clone encoding mOrange fluorescent proteinDepositorInsertmonomeric Orange fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C122-E14: mKate
Plasmid#162894PurposeStandard Gateway entry clone encoding mKate fluorescent proteinDepositorInsertmonomeric Kate fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C122-E10: mCherry
Plasmid#162892PurposeStandard Gateway entry clone encoding mCherry fluorescent proteinDepositorInsertmonomeric Cherry fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C122-E54: mRuby3
Plasmid#162899PurposeStandard Gateway entry clone encoding mRuby3 fluorescent proteinDepositorInsertmonomeric Ruby3 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVER-LacI
Plasmid#164830PurposeLacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.DepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterPtacAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTY1-LacI
Plasmid#164831PurposeLacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent proteinDepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterPtacAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB30-GFP
Plasmid#165481PurposepTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and HisDepositorTypeEmpty backboneUseTagsTEV-GFP-FLAG-10x HisExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1143
Plasmid#160869PurposePJ23110-RBS-RsVS-mRuby2-tL3S2P24_KanR_p15ADepositorInsertVinorine synthase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1142
Plasmid#160868PurposePJ23110-RBS-FvSAAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertAlcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1135
Plasmid#160866PurposePJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15ADepositorInsertChloramphenicol Acetyltransferase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23110_BBa_B0029Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1126
Plasmid#160858PurposePJ23110-RBS-FaAAT2-mRuby2-tL3S2P24_KanR_p15ADepositorInsertAlcohol acyl transferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG502-MAP4-frFAST
Plasmid#160058PurposeExpresses MAP4-frFAST in mammalian cellsDepositorInsertMAP4-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-delN-EcGlpG
Plasmid#158537PurposeBacterial expression of rhomboid protease EcGlpG without N terminusDepositorInsertdelta N-terminus EcGlpG
UseTagsGST tagExpressionBacterialMutationN-terminal deletion (1-86)PromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_FRT_GFP_12AAA_mCH
Plasmid#127111DepositorInsertGFP-mCherry fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_FRT_GFP_6TS_mCH
Plasmid#127110DepositorInsertGFP-mCherry fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI123
Plasmid#126063PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertGFP 11x3 (5 aa linker)
UseSynthetic Biology; Module 0TagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI124
Plasmid#126064PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertGFP 11x7 (5 aa linker)
UseSynthetic Biology; Module 0TagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI125
Plasmid#126065PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertYFP1-10
UseSynthetic BiologyTagslinker (GTGGGGS)ExpressionMutationT205YPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF2
Plasmid#107871PurposeBacterial expression of FGF2-NanoLucDepositorInsertFGF2
UseLuciferaseTagsNanoLucExpressionBacterialMutationwt sequencePromoterpro DAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodAcytCh-GFP-P4M
Plasmid#113346PurposeBacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)DepositorInsertsCherry
GFP-SidM-P4M domain (2 copies of P4M in tandem)
UseTagsExpressionBacterialMutationPromoterProD and proDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pre-Ubc4 BrG pUC19
Plasmid#111322Purposefor transcription to Ubc4 pre-mRNADepositorInsertUBC4 (UBC4 Budding Yeast)
UseTagsExpressionBacterialMutationintron has branch-site A to G mutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOBCol2.3MCSpA
Plasmid#109485PurposeDrive inserted gene in osteoblasts.DepositorTypeEmpty backboneUseTagsNoneExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal DsRedEx2
Plasmid#104024PurposePlasmid encoding 2ndVal DsRed-Express2DepositorInsert2ndVal DsRed-Express2
UseLow copyTagsExpressionBacterialMutationDsRed-Express2 with valine inserted in 2nd positi…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TM SE T206A
Plasmid#79664PurposeMore dimeric SE attached to Triple Mutant GEVI from Piao et al 2015DepositorInsertTriple Mutant SE T206A
UseTagsExpressionMammalianMutationT206APromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK593
Plasmid#71587PurposeProduces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE )
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS013
Plasmid#65466PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherryDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only