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We narrowed to 13,971 results for: EGFP

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Showing: 11851 - 11900 of 13971 results

  4. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  9. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  29. pLV-REPORT(EF1a)

    Plasmid
    #172326
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172326-plasmid-map-sequence-id-354701

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  30. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172327-plasmid-map-sequence-id-354684

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  32. pPgiCas13b-gRNA-1

    Plasmid
    #184834
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184834-plasmid-map-sequence-id-377030

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  34. pX330 _CRISPIE donor B8

    Plasmid
    #172849
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172849-plasmid-map-sequence-id-340928

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  35. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid
    #208573
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    d2nucEGFP
    HygroR
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK/CMV and minP
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208573-plasmid-map-sequence-id-412948

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  36. pX330 _CRISPIE donor B7

    Plasmid
    #172848
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172848-plasmid-map-sequence-id-341024

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  37. pX330 _CRISPIE donor B9

    Plasmid
    #172850
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172850-plasmid-map-sequence-id-341005

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  40. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid
    #208574
    Purpose
    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK/CMV and minP
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208574-plasmid-map-sequence-id-413226

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  41. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  43. pET15b-eGO-Matryoshka-LE-LP

    Plasmid
    #100017
    Purpose
    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpEGFP-LSSmOrange
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100017-plasmid-map-sequence-id-194293

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  44. pLV-REPORT(PGK)

    Plasmid
    #172328
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172328-plasmid-map-sequence-id-354688

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  47. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  48. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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Showing: 11851 - 11900 of 13971 results