We narrowed to 15,732 results for: LEA

1. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroX-PTuner DD-linker-M.EcoGII

   Plasmid

   #122082

   Purpose

   Express M.EcoGII with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of M.EcoGII

   Expression

   Bacterial and Mammalian

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a-Tn5

   Plasmid

   #127917

   Purpose

   Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Tn5
   

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIX402 PHF2

   Plasmid

   #91700

   Purpose

   inducible lentiviral expression of PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBCW

   Plasmid

   #62658

   Purpose

   Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3/Flag-WTAP

   Plasmid

   #53741

   Purpose

   For expression of WTAP (Wilms tumor 1 associated protein) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Wilms tumor 1 associated protein (WTAP Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVTNT-T7-KPNA4

   Plasmid

   #26680

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 4 (KPNA4 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Foxo1

    Plasmid

    #17551

    Depositor

    Domenico Accili

    Article

    Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

    Insert

    pEGFP-N1 Foxo1 (Foxo1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-KRAB-dCas9-IRES-GFP

    Plasmid

    #85556

    Purpose

    2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDS24

    Plasmid

    #58952

    Purpose

    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

    Insert

    Mitochondrially coded Arg8 protein
    

    Mutation

    Coding sequence synthetic in S. cerevisiae mitoch…

    Promoter

    n/a

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVTNT-T7-KPNA5

    Plasmid

    #26681

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 5 (KPNA5 Human)
    

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Egr1

    Plasmid

    #11729

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    Egr-1 (Egr1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRESneo-FLAG/HA Ago2 corrected

    Plasmid

    #10822

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    3 silent mutations in orf

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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