We narrowed to 12,214 results for: LEA
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Plasmid#29080PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable sinceAug. 3, 2011AvailabilityAcademic Institutions and Nonprofits only
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pHIV-EF1a-2A-ZsGreen
Plasmid#231985PurposeEmpty backbone for expression of GoI with Bi-cistronic expression ZsGreenDepositorInsert2A-ZsGreen
UseLentiviralAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-CGBE1
Plasmid#198537PurposeExpresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm5-mCer
Plasmid#211393Purposemammalian expression of human Mcm5 C-terminally fused to mCeruleanDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMcm5 (MCM5 Human)
TagsmCeruleanExpressionMammalianAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCherry F8-2
Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
TagsmCherryExpressionMammalianPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Z-AIMP1-1
Plasmid#166560PurposescFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
Tags6xHis - TEV cleavageExpressionBacterialPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9-p (with stop codon)
UseCRISPRAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GALTA-c001
Plasmid#79645PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEMS1113
Plasmid#29042PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEMS1265
Plasmid#29137PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1524
Plasmid#29255PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle53 (DCX Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceOct. 3, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-HSP90 (3O6O)
Plasmid#28153PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSP90
TagsHis tag with TEV cleavageExpressionBacterialMutationN-terminal domainAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBG-hBEN
Plasmid#22160DepositorAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-3
Plasmid#223224Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-eGFP-shAqp4-1
Plasmid#223223Purposemir30 based shRNA strategy, shRNA targeting mouse Aqp4DepositorInsertshAqp4 (Aqp4 Mouse)
UseAAVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only