We narrowed to 167,212 results for: addgene

Showing: 4741 - 4760 of 167212 results

Results per page:

Sort results by:

AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP1830

Plasmid

#70708

Purpose

Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Insert

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

CAG

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8e SpG Puro

Plasmid

#235044

Purpose

Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

ABE8e, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGDP3 aph(3'')-Ia

Plasmid

#112879

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-flex-GFP-4x6T

Plasmid

#196418

Purpose

Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

GFP

Use

AAV and Cre/Lox; Astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-kdrl:mCherry-caax

Plasmid

#194287

Purpose

Expression plasmid for zebrafish or Danionella cerebrum

Depositor

Pui-Ying Lam

Article

Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

Insert

kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)

Use

Zebrafish or danionella cerebrum expression

Tags

mCherryCAAX from the Tol2kit (Kwan et al., 2007)

Promoter

kdrl(flk1)

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ABCG1_pCSdest

Plasmid

#53952

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
p300 KAT LIC 1M

Plasmid

#233587

Purpose

Bacterial expression of p300 KAT enzyme

Depositor

Scott Showalter

Article

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300 KAT (EP300 Human)

Tags

6xHis MBP

Expression

Bacterial

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV PIG Pu.1

Plasmid

#66982

Purpose

Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Spi1 (Spi1 Mouse)

Use

Retroviral

Promoter

LTR

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE puro human IRS-1

Plasmid

#11025

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only