We narrowed to 9,265 results for: yeast expression
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Plasmid#193316PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
UseTags4MYCExpressionBacterialMutationPromoterAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193314PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
UseTags4MYCExpressionBacterialMutationPromoterAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArgininePromoterAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS420
Plasmid#11259DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS410
Plasmid#11258DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceFeb. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
JME4580
Plasmid#129659PurposeHPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleAvailable SinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB4695
Plasmid#171127PurposeA plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.DepositorInsertOsTIR1-F74A
UseOtherTagsExpressionMutationnon applicablePromoterAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
UseTagsExpressionInsectMutationPromoterAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLew100::NLS-ISceI-HA
Plasmid#21299DepositorAvailable SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
JME4599
Plasmid#129660PurposeNATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB5050
Plasmid#171126PurposeA plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.DepositorInsertOsTIR1
UseOtherTagsExpressionMutationnon applicablePromoterAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
JME4472
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-GFP-Ubc9ts
Plasmid#20369DepositorInsertSUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationY68L This mutation renders Ubc9 temperature-sens…PromoterAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
bRA90
Plasmid#100951PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
LHP306 - Ub - no Lys
Plasmid#32150DepositorUseTagsExpressionYeastMutationNo Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
JME4390
Plasmid#129656PurposeLEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only