We narrowed to 6,273 results for: nls
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Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorInsertBE3-FNLS
Tags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH275 NLS dTomato
Plasmid#168210PurposeMammalian expression of nuclear-localized dTomatoDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucDM-GFP
Plasmid#127796PurposeThe expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.DepositorInsertdestabilized mutant (DM) Luciferase
TagsEGFP and FLAG-NLSExpressionMammalianMutationR188Q, R261Q in Firefly Luciferase insertPromoterCMVAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS mPlum IX
Plasmid#37356DepositorInsertNLS mPlum
Available SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSPromoterRibo-HX1Available SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDDE488Q-pCDNA3.1(+)
Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-CD-DsRed
Plasmid#16345DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationFunctional Ca2+binding site CD was inactivated by…Available SinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA-19-NLS-4
Plasmid#55116PurposeLocalization: Nucleus, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1D
Plasmid#13284DepositorAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-TO-PV-NLS-Dsred
Plasmid#16341DepositorAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1/2D
Plasmid#13287DepositorInsertNPM (NPM1 Human)
TagsFlagExpressionMammalianMutationDeletion of aa134-aa142, aa150-aa155Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
mRuby-PCNA-19-NLS-4
Plasmid#55876PurposeLocalization: Nucleus, Excitation: 558, Emission: 605DepositorAvailable SinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLBS.CAG/NLS-mScarlet
Plasmid#129336Purposenucleus marker, lentivirusDepositorInsertmScarlet (Addgene 85042)
UseLentiviralAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
TagsNuclear localization signal (NLS)ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-nls-NgAgo-GK
Plasmid#86435PurposeExpresses a Flag tag on the N-terminalDepositorInsertNgAgo
TagsFlag tagExpressionMammalianPromoterCMVAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHeCAHS-mEGFP-NLS
Plasmid#205014PurposeThe vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.S_mCherry-NLS
Plasmid#178224PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.S and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-PCNA-19-SV40NLS-4
Plasmid#57994PurposeLocalization: Nucleus, Excitation: 555, Emission: 584DepositorAvailable SinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
RANLS-LEHD-BNES
Plasmid#60973PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
TagsCaspase9 substrate sequence (LEHD) is after NLS s…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-19-SV40NLS-4
Plasmid#55386PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-2C-NLS in pCSDest
Plasmid#126639PurposeExpresses FnoCas12a-2C-NLS in mammalian cellsDepositorInsertFnoCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianPromoterCMV IE94 promoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
RANLS-IETD-BNES
Plasmid#60972PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME tdTomato-NLS
Plasmid#135205PurposeRed fluorescent tagDepositorInserttdTomato
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR2
Plasmid#59012PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR2 genetically encoded Zn(II) sensor
TagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME nls-eos
Plasmid#73791PurposeMiddle entry clone with photoconvertible eos conjugated to a nuclear localization signalDepositorInsertnls-eos
UseMiddle entry cloneAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only