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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,191 results for: his

Showing: 921 - 960 of 22191 results

  1. EGF-bio-His

    Plasmid
    #53340
    Purpose
    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EGF (EGF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53340-plasmid-map-sequence-id-83070

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  4. CD34-bio-His

    Plasmid
    #51647
    Purpose
    Expresses full-length Hematopoietic progenitor cell antigen CD34 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD34 (CD34 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51647-plasmid-map-sequence-id-78970

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  6. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Use
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  8. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109121-plasmid-map-sequence-id-209767

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  11. PTGS1-bio-His

    Plasmid
    #53414
    Purpose
    Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTGS1 (PTGS1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53414-plasmid-map-sequence-id-83198

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  14. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

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  15. PTPRC-bio-His

    Plasmid
    #51813
    Purpose
    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRC (PTPRC Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51813-plasmid-map-sequence-id-79539

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  18. NOTCH3-bio-His

    Plasmid
    #53343
    Purpose
    Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    NOTCH3 (NOTCH3 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53343-plasmid-map-sequence-id-83073

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  31. pQE-60 roGFP2-His

    Plasmid
    #65046
    Purpose
    Bacterial expression of His-tagged roGFP2
    Depositor
    Tobias Dick
    Insert
    roGFP2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    see sequence
    Promoter
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65046-plasmid-map-sequence-id-350692

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  37. ITGAV-bio-His

    Plasmid
    #51919
    Purpose
    Expresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGAV (ITGAV Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51919-plasmid-map-sequence-id-79896

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Showing: 921 - 960 of 22191 results