Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,489 results for: dre

Showing: 1151 - 1200 of 5489 results

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pOCC114

Plasmid

#118904

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with TEV protease, two glycines

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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prCAG-RBS"m1"-sfGFP

Plasmid

#191400

Purpose

Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

47xCAG RBS"m1"-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLtet0-1

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSopB2xHA

Plasmid

#112513

Purpose

PsopB driven sopB-2xHA and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB-2xHA/sigE operon

Tags

2xHA

Expression

Bacterial

Mutation

SopB C-terminal 2xHA

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-1x

Plasmid

#124690

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pML104-kanR1136

Plasmid

#162040

Purpose

Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR1136 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRIV-Gal4DBD:2xnMagHigh1

Plasmid

#182031

Purpose

Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1-p103'UTR

Use

Donor plasmid

Expression

Insect

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-Pax7bait-T2a-Cherry-pA

Plasmid

#111154

Purpose

knock in bait for Axolotl Pax7 locus

Depositor

Elly Tanaka

Article

Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

Insert

Pax7bait-T2a-Cherry-pA

Expression

Mammalian

Mutation

wt

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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HisSumoOphP

Plasmid

#189672

Purpose

HisSUMO tagged OphP to express OphP in pichia pastorias.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

HisSumoOphP

Tags

HisSumo tagged

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMK110

Plasmid

#99535

Purpose

contains bgl operon sequence for E. coli in vitro transcription template

Depositor

Robert Landick

Article

Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

Insert

E. coli bgl antisense region containing bglG

Tags

None

Promoter

lambda Pr promoter used for in vitro transcriptio…

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK Mma PylT (UUA)

Plasmid

#154783

Purpose

plasmid with single copy of Mma PylT (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

U25C and UUA anticodon

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmycTurboCTD(C1)-PP1alpha

Plasmid

#253062

Purpose

Expression of mycTurboCTD-PP1alpha

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

PP1alpha (PPP1CA Human)

Tags

mycTurboCTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(N3)

Plasmid

#253059

Purpose

Contains a multiple cloning site to insert a gene upstream of Flag-TurboID (N-term aa1-194)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-NTD

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pmycTurboCTD(C1)

Plasmid

#253060

Purpose

Contains a multiple cloning site to insert a gene downstream of myc-TurboID (C-term aa195-320)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-CTD

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pmycTurboCTD(N3)

Plasmid

#253061

Purpose

Contains a multiple cloning site to insert a gene upstream of myc-TurboID (C-term aa195-320)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-CTD

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pmycTurboCTD(C1)-PP1gamma

Plasmid

#253064

Purpose

Expression of mycTurboCTD-PP1gamma

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

PP1gamma (PPP1CC Human)

Tags

mycTurboCTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(C1)

Plasmid

#253058

Purpose

Contains a multiple cloning site to insert a gene downstream of Flag-TurboID (N-term aa1-194)

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TurboID-NTD

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(C1)-NOM1

Plasmid

#253070

Purpose

Expression of FlagTurboNTD-NOM1

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

NOM1 (NOM1 Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(C1)-TPRN

Plasmid

#253069

Purpose

Expression of FlagTurboNTD-TPRN

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

TPRN (TPRN Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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GST-tag ARIH2 binding E2 variant L3A2-5

Plasmid

#248848

Purpose

N-term GST tag E2 variant selected for ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3A2-5

Tags

Avi and GST

Expression

Bacterial

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH2 binding E2 variant D3A2-1

Plasmid

#248849

Purpose

N-term GST tag E2 variant selected for ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-D3A2-1

Tags

Avi and GST

Expression

Bacterial

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH2 binding E2 variant L3A2-3

Plasmid

#248586

Purpose

N-term GST tag E2 variant selected for activated ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3A2-3

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH1 binding E2 variant L3A1-1

Plasmid

#248587

Purpose

N-term GST tag E2 variant selected for activated ARIH1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3A1-1

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag RNF14 binding E2 variant L3R14-1

Plasmid

#248589

Purpose

N-term GST tag E2 variant selected for RNF14

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3R14-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH1 binding E2 variant L3A1-2

Plasmid

#248854

Purpose

N-term GST tag E2 variant selected for ARIH1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3A1-2

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH2 binding E2 variant L3A2-2

Plasmid

#248846

Purpose

N-term GST tag E2 variant selected for ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3A2-2

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-tag ARIH2 binding E2 variant L3A2-4

Plasmid

#248847

Purpose

N-term GST tag E2 variant selected for ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3A2-4

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT5G07110-mKOk

Plasmid

#224850

Purpose

Plasmid for expression of AT5G07110.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G07110.1 (PRA1.B6 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT5G42765-mKOk

Plasmid

#224853

Purpose

Plasmid for expression of AT5G42765.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G42765.1 (AT5G42765 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT5G55530-mKOk

Plasmid

#224854

Purpose

Plasmid for expression of AT5G55530.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G55530.1 (AT5G55530 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT5G63060-mKOk

Plasmid

#224858

Purpose

Plasmid for expression of AT5G63060.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G63060.1 (AT5G63060 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT1G48590-mKOk

Plasmid

#224834

Purpose

Plasmid for expression of AT1G48590.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G48590.1 (AT1G48590 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT1G73430-mKOk

Plasmid

#224835

Purpose

Plasmid for expression of AT1G73430.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G73430.1 (AT1G73430 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT1G77730-mKOk

Plasmid

#224836

Purpose

Plasmid for expression of AT1G77730.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G77730.1 (AT1G77730 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT2G20590-mKOk

Plasmid

#224837

Purpose

Plasmid for expression of AT2G20590.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT2G20590.1 (AT2G20590 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT3G22620-mKOk

Plasmid

#224841

Purpose

Plasmid for expression of AT3G22620.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT3G22620.1 (AT3G22620 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT3G56110-mKOk

Plasmid

#224842

Purpose

Plasmid for expression of AT3G56110.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT3G56110.1 (PRA1.B1 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT4G00165-mKOk

Plasmid

#224843

Purpose

Plasmid for expression of AT4G00165.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT4G00165.1 (AT4G00165 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT4G11790-mKOk

Plasmid

#224844

Purpose

Plasmid for expression of AT4G11790.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT4G11790.1 (AT4G11790 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(k)-AT4G28430-mKOk

Plasmid

#224845

Purpose

Plasmid for expression of AT4G28430.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT4G28430.1 (AT4G28430 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUBC(b)-AT1G22850-mKOk

Plasmid

#224831

Purpose

Plasmid for expression of AT1G22850.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT1G22850.1 (AT1G22850 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_5a_007_mScarlet-I

Plasmid

#235889

Purpose

mScarlet-I C-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mScarlet-I C-tag

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_5a_006_MYC-tag

Plasmid

#235888

Purpose

MYC C-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

MYC C-tag

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PTPN20CP_pGCS1

Plasmid

#238500

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

PTPN20CP (PTPN20CP Human)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SRGAP2C_pEF-DEST51

Plasmid

#238497

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

SRGAP2C (SRGAP2C Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PDZK1P1_pGCS1

Plasmid

#238501

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

PDZK1P1 (PDZK1P1 Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ARHGAP11B_pEF-DEST51

Plasmid

#238498

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

ARHGAP11B (ARHGAP11B Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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