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Showing: 461 - 480 of 2948 results

  5. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  6. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

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  7. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  8. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  9. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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  11. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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  12. pFsynW SYT1 reporter

    Plasmid
    #197275
    Purpose
    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197275-plasmid-map-sequence-id-389085

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  13. pFsynW SYB2 reporter

    Plasmid
    #197276
    Purpose
    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYB2 and HaloTag (Vamp2 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197276-plasmid-map-sequence-id-389092

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  14. pFsynW SYT1deltaC2AB reporter

    Plasmid
    #197279
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197279-plasmid-map-sequence-id-389097

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Showing: 461 - 480 of 2948 results