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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Tags

Expression

Mutation

K24R- please see depositor comments

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-KIF3A-HL

Plasmid

#51397

Purpose

expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

KIF3A (Kif3a Mouse)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Use

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-ADRB2-mNG

Plasmid

#195145

Purpose

Image recycling cargo

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ADRB3 (ADRB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-YKT6

Plasmid

#116945

Purpose

Expressing Myc-YKT6 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

Synaptobrevin homolog YKT6 (YKT6 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A_no_ccDB_mUty

Plasmid

#158570

Purpose

V5-tagged sequence of mUty cloned into entry vector for recombination

Depositor

Christian Deschepper

Article

Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

Insert

Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_NEMF

Plasmid

#127126

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA NEMF (NEMF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAtipiTrc

Plasmid

#53275

Purpose

Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

Insert

ipi1 (IPP2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138/139R

Plasmid

#127138

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Tags

Expression

Mutation

K138R/K139R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Desmocollin2_tail-GST

Plasmid

#36992

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsc2 (DSC2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of Dsc2 (nt 2,603–3,158)

Promoter

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
Smp_090100-Cd4-bio-His

Plasmid

#120655

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_090100 (Smp_090100 Schistosoma mansoni)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RNF123

Plasmid

#127122

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RNF123 (RNF123 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Smp_112090-Cd4-bio-His

Plasmid

#120662

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_112090 (Smp_112090 Schistosoma mansoni)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Smp_019000-Cd4-bio-His

Plasmid

#120704

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_019000 (Smp_019000.1 Schistosoma mansoni)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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VCDstub-GFP

Plasmid

#33122

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT190-GFP

Plasmid

#33118

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 190 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

Plasmid

#231601

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

Plasmid

#231603

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA

Plasmid

#231604

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge