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We narrowed to 2,059 results for: gem

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Showing: 461 - 480 of 2059 results

  7. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  9. pGEMHE-MmCENPT-4NS-GFP

    Plasmid
    #237431
    Purpose
    Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 T to A; 463 I to T, 470 L to E, 485 Q to L
    Promoter
    T7
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    237431-plasmid-map-sequence-id-474223

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  10. pGEMHE-MmCENPT-ASD2HFD-GFP

    Plasmid
    #237430
    Purpose
    Expresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with ancestral HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from mouse:rat anc…
    Promoter
    T7
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237430-plasmid-map-sequence-id-474256

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  11. pGEMHE-MmCENPT-RpHFD-GFP

    Plasmid
    #237428
    Purpose
    Expresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rhabdomys pumilio HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Rhabdomys pum…
    Promoter
    T7
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237428-plasmid-map-sequence-id-474254

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  12. pGEMHE-MmCENPT-McHFD-GFP

    Plasmid
    #237437
    Purpose
    Expresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Mus caroli HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Mus caroli
    Promoter
    T7
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237437-plasmid-map-sequence-id-474253

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  13. pGEMHE-MmCENPT-RnHFD-GFP

    Plasmid
    #237429
    Purpose
    Expresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Rattus norveg…
    Promoter
    T7
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237429-plasmid-map-sequence-id-474225

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  14. pGEMHE-MmCENPT-MsHFD-GFP

    Plasmid
    #237427
    Purpose
    Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Publication
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Mus spretus
    Promoter
    T7
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237427-plasmid-map-sequence-id-474221

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  15. EP003: CAG::GEM7-HaloTag

    Plasmid
    #209303
    Purpose
    Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209303-plasmid-map-sequence-id-414428

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  17. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

    Plasmid
    #225436
    Purpose
    BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.
    Depositor
    Diego Orzaez
    Publication
    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1
    Insert
    BeYDV geminino 1.0
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225436-plasmid-map-sequence-id-447735

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Showing: 461 - 480 of 2059 results